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malP [2019-07-25 09:41:49]
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malP [2019-07-25 09:41:49]

maltose-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIICB of the [category|SW 1.2.2|PTS]
locus
BSU_08200
pI
8.87
mw
57.89 kDa
protein length
527 aa Sequence Blast
gene length
1584 bp Sequence Blast
function
maltose uptake and phosphorylation
product
maltose-specific [category|SW 1.2.2|PTS], EIICB
essential
no
ec
2.7.1.69
synonyms
glvC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    892,215 → 893,798

    Phenotypes of a mutant

  • no growth on maltose as single carbon source [Pubmed|10627040]

    • The protein

    Catalyzed reaction/ biological activity

  • Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

    • Protein family

  • [category|SW 1.2.2|PTS] permease, glucose family [Pubmed|10627040]

    • Structure

  • [PDB|6BVG] (EIIC domain from B. cereus, corresponds to aa 6 - 419, 27% identity) [pubmed|29784777]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
    description
    [Pubmed|11489864]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489864], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR]: activation, [Pubmed|11489864], in [regulon|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|11489864], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C291 (glvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2289 NBRP B. subtilis, Japan]
  • GP110 (spc), available in [SW|Stülke] lab
  • BKE08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
  • BKK08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC, downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC

    • References

  • 16707683,10627040,11489864,30038046,29784777